Question

Consider the following short peptide: Ala-Gly-Lys-Ser-Asp What is the difference in the number of primary amines...

Consider the following short peptide:

Ala-Gly-Lys-Ser-Asp

What is the difference in the number of primary amines this peptide would yield after it was fully hydrolyzed versus when it was intact as shown (report the absolute/positive value)?

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Answer #1

Peptide is consisting of amino acids and it is also called poly amide. Its structure start from N-terminus to end C-terminus.Amino acid has two functional properties, one amine (primary) and second carboxylic acid.

Primary amine is characterised by -NH2.

In this peptide, -NH2 group means N-terminus free and lysine -NH2 free.

But after hydrolysis, Acidic, each amide bond will be cleaved and willproduce Amini acid, therefore each -NH2 will be frree and in HClit willbe in ammonium salt and one -NH2 more from lysine.

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