Question

Given stock solutions of glucose (1.00M), asparagine (0.100M), and NaH2PO4 (0.0500 M), explain how you would...

Given stock solutions of glucose (1.00M), asparagine (0.100M), and NaH2PO4 (0.0500 M), explain how you would prepare 500 mL of a reagent that contains 50.0 mM glucose,10.0 mM asparagines, and 2.00 mM NaH2PO4

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Homework Answers

Answer #1

We can use dilution method to prepare the solution by using

M1V1 = M2V2

M1V1 = molarity and volume of stock solution and M2V2 = molarity and volume of dilute solution

GLUCOSE

1.0 X V1 = 0.05 X 500, 50mM = 50 / 1000 = 0.05M

V1 = 25mL

asparagine

M1V1 = M2V

0.1 X V1 = 0.01 X 500

V1 = 50mL

NaH2PO4

M1V1 = M2V2

0.05 X V1 = 0.002 X 500 = 20mL

Total volume of concntratd solutions = 25mL + 50mL + 20mL = 95mL

Mix these stock solution to get a total of 95mL and then dilute by adding 405mL of distilled water to make the desired solution.

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