Question

How can I test the validity of Beers law given a range of sample absorbance values measured from a spectrometer?

​I have 6 absorbance values for CuSO₄​, and their absorbance - And I need to produce a formal lab with a test of the validity of Beer's law. How can I perform such a test?

Answer 1

Beers law or Lambert - Beers law mathematically defined as-

A = cl, Where A = Absorbance, = Extinction coefficient, c = Concentration of Analyte and l = length of path length

If you are going to plot the graph between Absorbance (A) and Concentration (c) of analyte then it will be a straight line having the slope l.

To test the validity of Beers law, you just need to plot the graph above mentioned and if the graph is a straight line then it will tell that the Beers law is valid and if it deviates from the straight line behaviour then you can say that the Beers law is not valid.

Note: Generally, Beers law holds true in case of very dilute solutions (in the concentration range of M solutions) because in dilute solutions there is negligible mutual interactions but they became pronounce in concentrated solutions that's why the graph between A and c deviates from ideal behaviour.