Question

Which technique listed below has the best DL for folic acid given the values for the DL shown here? Circle the correct answer.

HPLC, with a DL = 1.8 μg/mL;

Fluorescence, with a DL = 0.50 μg/mL;

Absorbance, with a DL= 2.6 μg/mL.

Answer 1

Ans. “Detection limit is the lowest concentration of the analyte that can be detected”.

The smaller the DL, the better.

# For example, an UV-vis spectrophotometer has a DL of 100.0 ug/mL for a hypothetical analyte.

HPLC has the DL of 1.8 ug/mL for the same analyte.

If a solution has [analyte] = 10.0 ug/ mL , the UV-vis spectrophotometer fails to detect the analyte in the sample. However, HPLC can detect it .

# Therefore, the smaller the DL, the better.

So, correct option is- Fluorescence, with a DL = 0.50 ug/ mL