Question

Which of the following best describes Pulsed-field Gel electrophoresis? It is used for surveillance and investigation...

Which of the following best describes Pulsed-field Gel electrophoresis?

  • It is used for surveillance and investigation of foodborne illness outbreaks.
  • It is the result of frequency mapping.
  • It is used only when larger DNA fragments stop the pulsing electric currents.
  • All of the above describe Pulsed-field Gel electrophoresis.

The PFGE samples examined by Lanni show similar illnesses clustered in which one of the following states?

  • Oregon
  • Washington
  • Nevada
  • California

Which of the following is included in the case definition of this outbreak?

  • Clinical information
  • Employers
  • Specific residence
  • Educational level

Based on what has been presented thus far, what type of outbreak has occurred?

  • Common-source outbreak
  • Point- source outbreak
  • Continual-source outbreak
  • None of the above

What age group is most affected by this outbreak?

  • Individuals over 55
  • Individuals under 55
  • Individuals between 25 and 35
  • Individuals between 10 and 18

Which of the following PFGE patterns does not match the current outbreak?

  • Both are correct
  • None of the above

What is the best control measure to take at this time?

  • Exclude people at high risk from work, school, daycare, etc.
  • Host conference calls with public health agencies.
  • Remove contaminated food.
  • Do none of the above.

Based on the information presented thus far, which of the following best describes this outbreak?

  • Most of the individuals affected have low blood pressure and high rates.
  • Most of the individuals affected have diarrhea.
  • Most of the individuals affected have a rash.
  • Most of the individuals affected have high fevers.

Homework Answers

Answer #1

Answer to the first question is fourth option. All of the statements describe the pulse-field gel electrophoresis. It is used for surveillance and investigation of foodborne illness, it is result of frequency mapping and it is used only when the large DNA molecule stop the pulsing electric currents. Pulse field electrophoresis is used to separate large DNA molecules and it differs from normal electrophoresis as the direction of the current flowing through the gel matrix changes periodically between 3 directions with each electrode located 60 degrees apart.

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