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Discuss why a standard curve is required to measure the unknown protein concentration.

Discuss why a standard curve is required to measure the unknown protein concentration.

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Answer #1

How standard curve drawn: (protein concentration vs absorbance)

Multiple samples with known properties are measured and graphed (standard curve), and same properties to be determined for unknown samples by interpolation on the graph.

Determining the concentration of a protein in an unknown sample by comparing the unknown to a set of standard samples of known concentration

The standard curve is required for measuring unknown protein concentration in a simple, sensitive and accurate manner.

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