Question

1. Major steps in PCR.
2. Define Intron and Axon.
3. Master mix in PCR.
4. Function of Primer.

Answer 1

1. Their are 3 steps in PCR

a. Denaturation

b. Annealing

c. Extension

2. Introns and exons are nucleotide sequences within a gene. Introns are removed by RNA splicing as RNA matures. while exons go on to be covalently bonded to one another in order to create mature mRNA.

3. A master mix usually contains a thermostable DNA polymerase, dNTPs, MgCl2, and proprietary additives in a buffer optimized for PCR

4. PCR primers are short fragments of single stranded DNA (15-30 nucleotides in length) complementary to DNA sequences that flank the target region of interest