Question

Determination of LDH in Extracted Samples: Is it important to mix quickly and to read the...

Determination of LDH in Extracted Samples:

Is it important to mix quickly and to read the first measurement quickly, or is it better to wait? Why or why not?

Science   Biology   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

Ans . LDH (lactate dehydrogenase) test measure the amount of LDH in the blood. LDH is found in many of the body's tissues and organs , including the muscles, liver, heart, pancreas, kidneys, brain and blood cells. The LDH test is mainly used to help identify the location and severity of tissue damage in the body.

LDH is a stable enzyme, present in all cell types, which is rapidly released into the cell culture medium upon damage of the plasma membrane. LDH is the most widely used marker to run a cytotoxicity.

The method of LDH is based on flurometric determination of enzyme kinetics when L - lactate is converted to pyruvate.

A substrate concentration of 35mM in the reaction mixture was considered to be optimal for the LDH assay.

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
Why is the use of representative samples especially important in frequency claims? A. Frequency claims require...
Why is the use of representative samples especially important in frequency claims? A. Frequency claims require very large samples, and representative samples are always large. B. It is unethical to make frequency claims without representative samples. C. It is unlikely that the accuracy of estimates can be checked. D. Representative samples allow for enhanced internal and external validity. Which of the following is true when asking people questions about themselves? A. The confidence people have in their memories is not...
Read the following case and answer the questions that follow. Can employees really manage themselves? At...
Read the following case and answer the questions that follow. Can employees really manage themselves? At W.L. Gore and Associates, self-managed teams have helped create a thriving business that has operated profitably for more than 50 years. Gore is a manufacturer that develops innovative solutions for demanding environments. Focusing primarily on protective fabrics, Gore products might be found in clothing worn on a hike up Mt. Everest or in medical implants for the human body. You may have encountered their...
QUESTION 1 (60) Read the case study below and answer the questions that follow. Transforming a...
QUESTION 1 (60) Read the case study below and answer the questions that follow. Transforming a pharmacy together: the Charlotte Maxeke Johannesburg Academic hospital. Before 2015, the patient experience at the Charlotte Maxeke Johannesburg Academic Hospital pharmacy went something like this: take the day off work to have your prescription filled; line up at dawn before the pharmacy opens in hopes of beating the rush; once inside, wait up to several hours for your prescription to be filled; or worse,...
Read and comment: $2500: “A Fair Price for Hamilton” NY Times, 23 October 2016 Gregory Mankiw...
Read and comment: $2500: “A Fair Price for Hamilton” NY Times, 23 October 2016 Gregory Mankiw Consumers of goods and services do not typically wish that producers charged higher prices. But that was exactly my desire on a recent trip to New York City. The story begins with a basic mismatch: I am a big fan of theater, and I live just outside Boston. While Boston is a good city for the arts, it is not the mecca that New...
Read the Who makes the Apple iPhone? case study and answer the question which follows. In...
Read the Who makes the Apple iPhone? case study and answer the question which follows. In answering the question, relate your discussion to the facts presented within the case. Who makes the Apple iPhone? In its early days, US-Based Apple usually didn’t look beyond its own backyard to manufacture its devices. By 2004, however, Apple had largely turned to foreign manufacturing. The shift to offshore manufacturing reached its peak with the iconic iPhone, which Apple first introduced in 2007. All...
Why does the infinity reaction not require an infinite amount of time? PROCEDURE: Prepare 60 mL...
Why does the infinity reaction not require an infinite amount of time? PROCEDURE: Prepare 60 mL of a 1:1 (by volume) solution of distilled water and 2-propanol in a beaker. Be as precise as possible. After mixing well, wait 10-15 minutes to allow the contents to come to room temperature as some heat will evolve during the mixing of the two liquids. Obtain ~20 mL of 0.05M NaOH and put it in a small plastic storage bottle. Set up a...
Lab 9 – Molecular Biology In this lab, you will prepare an agarose gel and use...
Lab 9 – Molecular Biology In this lab, you will prepare an agarose gel and use gel electrophoresis to compare the size of 2 dye molecules Methyl orange and Ponceau G. You will also analyze an “unknown” sample that contains a mixture of two dyes. Dye molecules with lower molecular weight or greater electrical charge will migrate faster through the gel, than dye molecules with greater molecular weights or lesser electrical charge. Materials: Mini gel electrophoresis chamber 6 Tooth comb...
READ THE CASE STUDY AND ANSWER THE FOLLOWING QUESTIONS 2nd CASE: An Unexplained Death A 65-year-old...
READ THE CASE STUDY AND ANSWER THE FOLLOWING QUESTIONS 2nd CASE: An Unexplained Death A 65-year-old man of Scandinavian descent was rushed to the Emergency Room of your local hospital after a family member discovered him unconscious in his home. The woman who dialed “911” told the dispatcher that the man, her brother, was the local librarian of the past 10 years and had no spouse or children. She reported that they had spoken the day before, and he had...
read article below, give me your thoughts, 4 sentences. ty re you ready to interview your...
read article below, give me your thoughts, 4 sentences. ty re you ready to interview your interviewer? Candidates usually arrive at an interview ready to articulate their strengths and discuss their resume. However, it’s equally important to prepare to gather information and perspective in order to thoroughly evaluate the job. The questions you ask will provide you with the chance to showcase your knowledge of the company, your thoroughness, and motivation to secure the position. Add to that the opportunity...
What happened on Lebanon economics? Read this Articals and share with us your opinion and solution....
What happened on Lebanon economics? Read this Articals and share with us your opinion and solution. A Lebanese anti-government protester holds a placard as she sits for a haircut in front of the central bank headquarters in Beirut to protest against the economic policies of the bank. (AFP) A gathering of Lebanese anti-government protesters getting haircuts in front of the central bank building in Beirut last week attracted many jokes and much amusement. The protesters were expressing their rejection of...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT