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2. Two student groups, labeled Group 1 and Group 2, performed the gDNA isolation protocol incorrectly....

2. Two student groups, labeled Group 1 and Group 2, performed the gDNA isolation protocol incorrectly. Group 3 performed the gDNA isolation protocol correctly.

a. Group 1 treated the HTL lysate with resin before they added nuclease but followed all other steps. What would be the expected DNA concentration and purity for Group 1 as compared to Group 3?

b. Group 2 forgot to add nuclease but otherwise completed followed all other steps. What would be the expected DNA concentration and purity for Group 2 as compared to Group 3?

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Answer #1

Nuclease is the enzymes that digest DNA or RNA into nucleotides. RNase digest only RNA and DNase digest only DNA.

A - Given that group 1 added nuclease while isolating the genomic DNA. They will get a smear on the gel and their DNA concentration will be very less as compared to group 3.

Given that group 2 forget to add nuclease while isolating the genomic DNA. The cell has RNA along with DNA. So, RNA will not be digested. When we measure the concentration of DNA, due to contamination of RNA, we will get more concentration.

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