Question

. Assume that you have collected 35 ml egg white. Answer the following questions: a) How...

. Assume that you have collected 35 ml egg white. Answer the following questions: a) How much 1.0 N Acetic acid will be added to the above egg white? What is the purpose of adding acetic acid? Explain briefly. b) What will be the pH of the egg white solution after adding 1 N acetic acid, assuming egg white is neutral?

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Answer #1

Answer: The acetic acid is used to acidify the egg white due to which it becomes easy to break the protein bonds. It alters the electrostatic properties and causes denaturation of the proteins by breaking various bonds between the amino acids. The quantity of the acetic acid to be added will be based on the pH of the solution because acetic acid is used to reach the pH at isoelectric point. The required pH for the albumin purification is 6.8- 7.0 so the pH will range between them after adding the acetic acid

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