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Why do we want to exclude water from a Simple Structure-Activity-Relationship (SAR) Study Using Chemiluminescent Esters

Why do we want to exclude water from a Simple Structure-Activity-Relationship (SAR) Study Using Chemiluminescent Esters

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SAR studies the differences in enzymatic activities of an enzyme towards various electron-withdrawing or donating groups. This is done by the measurement of chemiluminescence to study structure-function relationships. However, since this method relies on the electron-withdrawing and donating properties, water could interfere with the process as water is a nucleophile, or electron donor. So, the SAR study requires all the tubes to be dried well to remove interference from water molecules.

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