Question

The melting temperature, TmeltTmelt, is defined as the temperature where ff, the fraction of single strands in the helix at equilibrium, is 1/2. Calculate the melting temperature for the following double helix

5'-GGGCCC-3'
3'-CCCGGG-5'

at 1.0×10−4M1.0×10−4M initial concentration of each single strand (the initial concentration, cc, is 1.0×10−4M1.0×10−4M).

Express your answer as an integer.

TmeltTmelt =

∘∘C

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Part H

The melting temperature, TmeltTmelt, is defined as the temperature where ff, the fraction of single strands in the helix at equilibrium, is 1/2. Calculate the melting temperature for the following double helix

5'-GGTTCC-3'
3'-CCAAGG-5'

at 1.0×10−4M1.0×10−4M initial concentration of each single strand (the initial concentration, cc, is 2.0×10−4M2.0×10−4M). Note that the oligonucleotides in the double helix are not self-complementary.

Express your answer as an integer.

TmeltTmelt =

31

∘∘C

Answer 1

Marmur Doty formula is used to calculate the melting temperature () for sequences that are 14 bases or less:

Where, Tm = melting temperature in °C
A = number of adenosine nucleotides in the sequence
T = number of thymidine nucleotides in the sequence
C = number of cytidine nucleotides in the sequence
G = number of guanosine nucleotides in the sequence

Therefore, for this sequence 5'-GGTTCC-3'