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You are trying to purify PFK-1 from a rat liver. Ideally, what should happen (increase, decrease...

You are trying to purify PFK-1 from a rat liver. Ideally, what should happen (increase, decrease or stay constant) to total protein, PFK-1 activity, and specific activity during a purification procedure of PFK-1?

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Answer #1

The PFK-1 (Phospho Fructo Kinase-1) it catalyzes the transfer of the pohosphate group from the ATP to the Fructose and produces Fructose-6-Phosphate.This enzyme has great impact on the glycolytic pathway.

So when purifying this PFK-1 from the rat liver the total protein content will be unchanged or stay constant,as the PFK-1 does not have any effect in the proteins.

The activity of the PFK-1 depends on the ATP/AMP ratio,the activity of the PFK-1 is inversely related with the ATP/AMP ratio.So at the time of purification the ratio of ATP/AMP is generally low in concentration,so the activity of the PFK-1 may increase,now at this point if the activity gets increased there is a possibity lies there that the specific activity will also increase because the specific activity is the activity of an enzyme per miligram of total protein.

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