Question

Q1) The RNAi pathway can serve as a transposable element suppression mechanism by degrading

  
A.the double stranded DNA of active transposable elements

   
B. both the double stranded DNA and transposase enzymes of active transposable elements

   
C. the transposase enzymes produced by active transposable elements

   
D. the mRNAs produced by active transposable elements

Q2) Which of the following are methods of reverse genetics?

A. phenocopying only
B. phenocopying, targeted mutagenesis and phylogenetic analysis
   C. phenocopying and targeted mutagenesis
D phylogenetic analysis only
E. targeted mutagenesis only

Answer 1

Please find the answers below:

Answer 1: Choice D (RNA interference is a technique which utilizes silencing and subsequent degradation of target mRNA of any origin. Hence, this technique can be used to silence a transposable element by degrading the mRNA generated by the transposon)

Answer 2: Choice B (Reverese genetics makes use of techniques which are based upon identification of a phenotype or expression starting from the lowermost element responsible for it. This means that techniques related to genetic manipulation such as random or targetted mutagenesis, phenocopying and reverse phylogenetic analysis are parts of reverse genetics)