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What are the limitations to Culturing and Differentiation of Small Intestinal Organoids techniques? Does it fully...

What are the limitations to Culturing and Differentiation of Small Intestinal Organoids techniques? Does it fully represent in vivo tissue? How can the technique be improved?

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Answer #1
  1. The culturing of cells and tissues is an easy task when comparing with the culturing of an whole oragn in an artifical medium environment.
  2. The organ culture involves development from the tissue culture method.The whole organ cannot be cultured requiring high amount of oxygen but peices of organs can be cultured in artifical medium .
  3. There are grift and raft methods along with organ culture in liquid medium can be followed to grow and understand the differentation and other mechainsm related.
  4. There are various limitations to it as results obtained are not comparable with whole orgainsm study,along with ethical limitations.
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