Question

Can some one code this in biopython.

brca1.txt is a normal gene- tca tgg tgg gtg agg ggt cag ctg gtg ggg cac aga tgc gtt tcc tcg gca gtc cac ata ttc ata gct ctg aaa gac cag ctg ctc tga atg gct cag gta tga aca ggt cag ggt gcc

, brca1&2.txt (modified gene)n- tcT a tgG g tgg gtg agg ggt cag ctg gtg ggg cac aga tgc gtt tcc tcg gca gtc cac ata ttc ata gct ctg aaa gac cag ctg ctc tga atg gct cag gta tga acG a gG gt cag ggt gcc

. If there is mutation in multiple places. How do we navigate the location. Please help.

ANSWER should look like

Location is 3, 7, 90,93

Answer 1

Mutation is the process of change in the nucleotide sequence due to many regions. There is many methods can be used to nevigate the location of mutation. These are following:

1. real-time PCR: It is a type of PCR, which give results during amplification. It will produce a fluorescent compound during a match but nothing will produce in mismatch.

2. Qfluorescent PCR (QF-PCR): This type of PCR also used to quantify any gene on the basis of their Ct value.

3. Multiplex ligation-dependent probe amplification (MLPA): It also work on PCR principle, where it use single primer to amplification.

4. Microarray: This technique is highly used for detection of the DNA or RNA sequence.