Question

You were recently bitten by a werewolf while on backpacking along the moors of Yorkshire. Anecdotal evidence suggests that the transition into a lupine beast is caused by a set of RNA molecules that are thought to be very lowly expressed, non-polyadenylated, and very large (>500,000 nt). You want to sequence the werewolf RNA molecules to help find a cure but don’t have any information other than what you’ve been told here and in class (and I guess stuff you find on the internet). Describe the approach you might take to isolate and sequence the werewolf RNA. Be sure select a process that will limit your need to sequence RNA molecules unimportant to your search for a cure to your problem.

Answer 1

Answer- The bitten area can be swabed to get a saliva sample of the werewolf. The genetic material can be extracted from the saliva with the organic extraction method. The sample should be processed differently from both of the RNA and DNA. If RNA is extracted directly then it should be electophorised first to observe a band of 500,000 nt or bigger and then the band should be amplified by amplification procedure and then it's sequencing should be performed to get the target sequence.