Question

1. Yeast were mutated and four haploid strains were found to that could not grow on...

1. Yeast were mutated and four haploid strains were found to that could not grow on minimal media (MM). What is minimal media? Can wild-type yeast grow on minimal media?

2. The mutant strains in #1 were able to grow on minimal media only if it was supplemented with urea. Explain what this tells you about the mutant strains.

Science   Biology   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

Minimal media contains basic components required for the growth of WT strain. It contains all the precursors required for the growth and proper development of the WT strain.

If a mutant can grow in a media only when supplemented with a nutrient, it shows that the mutant is unable to convert a precursor present in the minimal media to the supplemented metabolite.

Yes. WT strains can grow on minimal media.

It is given that a mutant strain can grow only in the presence of urea. This shows that the mutant strain can not convert a precursor present in the minimal media to urea. So, it is an auxotrophic strain for urea.

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
The yeast Saccharomyces cerevisiae can grow as haploid or diploid cells. You have two haploid yeast...
The yeast Saccharomyces cerevisiae can grow as haploid or diploid cells. You have two haploid yeast strains that each carry mutations that make them unable to grow when galactose is the sole available source of energy. One strain has a deletion of the entire region of the genome between the GAL1 and GAL10 genes (DUAS). The other strain carries a mutant allele of GAL2 that produces no functional GAL2 protein. You cross these two strains together to get diploid progeny....
You are working with three mutant strains of yeast. One mutant lacks glyceraldehyde-3-phosphate dehydrogenase, one lacks...
You are working with three mutant strains of yeast. One mutant lacks glyceraldehyde-3-phosphate dehydrogenase, one lacks alcohol dehydrogenase, and the third lacks pyruvate dehydrogenase. One of the three mutant strains above is able to grow on glucose under both anaerobic and aerobic conditions, although it grows somewhat slower than the wild type (normal) strain under aerobic conditions. Which strain is this? Explain your reasoning.
3. A number of different mutant yeast strains were isolated (1-5), all of which needed compound...
3. A number of different mutant yeast strains were isolated (1-5), all of which needed compound G for survival. While the precursors to G are known (compounds A-E), investigators did not know their order in the pathway. To determine this, each compound was tested for its ability to support growth for each mutant. The results of this experiment are shown below. A “+” sign indicates that the chemical was able support growth, but a “–“ indicates that it was not...
1. Currently you are studying threonine synthesis in E. coli. To find genes whose protein products...
1. Currently you are studying threonine synthesis in E. coli. To find genes whose protein products are important for threonine synthesis, you perform a mutagenesis to find mutants that require a source of threonine to grow. (a) Immediately after mutagenizing the bacteria, would you choose to grow the bacteria on rich media or on minimal media? Briefly explain your choice. Rich media. A mutant unable to make a particular amino acid will not be able to grown on media lacking...
(True Story) In 1952, the world’s first nuclear reactor meltdown took place at Canada’s Chalk River...
(True Story) In 1952, the world’s first nuclear reactor meltdown took place at Canada’s Chalk River experimental nuclear station, with a significant release of radioactivity to the surrounding environment. (A U.S. Navy nuclear Engineer named Jimmy Carter participated in the cleanup). As part of a study of the biological effects of the meltdown, microbiologists isolated E.coli strains for analysis. Eventually, one of them was found to have a mutation in the gene encoding a tRNA-tyr. The anticodon of this tyr...
2. In ancient days, a tribe of natives on the mythical continent of Atlantis were able...
2. In ancient days, a tribe of natives on the mythical continent of Atlantis were able to produce two commodities to eat. They could harvest fish from the sea and they could grow a form of wild oats. Table 5 and Graph 1.a. both show the maximum annual output combinations of fish and wild oats that could be produced by the natives of Atlantis. Table 5 Maximum annual output options Kilograms of fish Bushels of wild oats 1 7,000 0...
Activity 1: Scientific Reports You may have heard the question “If a tree falls in a...
Activity 1: Scientific Reports You may have heard the question “If a tree falls in a forest and no one is around to hear it, does it make a sound?” A similar question can be asked about experiments. “If a researcher performs an experiment and never publishes the result has science been performed?” Many people would say no because science is the accumulation of knowledge. If the results of an experiment are not published, knowledge is not gained. The final...
Question 1 As companies grow in size, it is inevitable for the shareholders to hire management...
Question 1 As companies grow in size, it is inevitable for the shareholders to hire management to run the operations of the business. The entire team of management, starting from the CEO and other top-level management, all the way to the middle and bottom level management are expected to perform towards the growth of the business. Since the shareholders of large companies are scattered across geographies, they appoint certain members as representatives who are elected to represent them on the...
Choose the false statement: A. If cellular respiration were to stop, as occurs in the absence...
Choose the false statement: A. If cellular respiration were to stop, as occurs in the absence of oxygen or presence of cyanide, the amount of ATP stored in the cell could keep it alive for 1-2 hours. B. Cells that require more energy to function, such as muscle or liver cells, have many more mitochondria than those requiring less energy, like RBC’s or skin cells. C. The purpose of alcoholic fermentation by yeast cells is to re-oxidize the NADH back...
Answer any FOUR of the following questions. Each question is worth a total of 12.5 marks....
Answer any FOUR of the following questions. Each question is worth a total of 12.5 marks. If more than four questions are answered, marks will be awarded for the first four answers only. Question 1 Nataliya is a fan of soy yoghurts, but recently she has noticed less soy yoghurts on supermarket shelves, with new alternative products such as almond milk yoghurt appearing in their place. What effect does the entrance of new firms have on the market for almond...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT