Question

Here’s an uncompleted table of a segment of the transcribed region of a gene in a prokaryote. Fill in the empty slots of this table with the appropriate nucleotide/amino acid based on the information you have here. (This requires that you use your awesome knowledge from previous weeks in this unit as well, but you can do it! I know this is challenging, but it’s not impossible. You have enough information here to crack this thing wide open.)

DNA double helix

strand #1

A

A

T

strand #2

A

C

G

mRNA sequence

U

G

U

tRNA anticodon

U

U

amino acid

threonine/thr

glutamate/glu

a) Well now that you’ve filled in that chart, which of the two DNA strands (strand #1 or strand #2) is the coding strand?

b) Aaamaaazing! How did you know that/what piece of information from the chart told you that?

Answer 1

DNA double helix str.

strand 1# coding strand seq. 5' AAGTGGCTTCTCAGTCGT 3'

strand 2 # template strand seq. 3' TTCACCGAAGAGTCAGCA 5'

mRNA seq. 5' AAGUGGCUUCUCAGUCGU 3'

anticodon seq. of tRNA 3' UUC/ACC/GAA/GAG/UCA/GCA 5'

amino acid seq. phenylalanine/threonine/glutamate/glutamate/serine/alanine

2). the given nucleotide and amino acid sequence give an idea about to suppose the idea of nucleotide seq in tRNA and from that we draw nucleotide seq of mRNA and ultimate double helix str. of DNA.