Question

Imagine that you are designing a scaffold for cartilage tissue engineering? Describe the requirements of engineered scaffolds for cartilage tissue engineering application. How would you manufacture that desired scaffold? Briefly explain manufacturing method. You will have to use a proper bioreactor system to increase adaptivity of cartilage scaffold to in vivo conditions. Please define what type of bioreactor you need to use. What are the demands that tissue engineering bioreactors have to meet? Would you prefer static or dynamic cell seeding and why?

Answer 1

Regenerative medicine and tissue engineering has brought before us a new spectrum of fusion between science and technology. Scaffolds play a very crucial role in the field of tissue engineering. Scaffolds help the organ to stay in place and helps in the process of regeneration. In simple words, with the help of scaffolds the organs retain back it's original biological structure and function. Scaffolds are manufactured in laboratories using the laser aided gelling technology, porogen leaching and textile technology. The main concern during scaffold manufacturing is its effectiveness in biodegradability. We need to use a proper bioreactor in order to allow cell cultivation and cell proliferation. Apart from this, there is also a need for nutrient dispersal which is possible by the bioreactor. We need to use a bioreactor with dynamic cell seeding.