Experiment 2: Spectrophotometry 1. Fill in the following table: Wavelength (nm) Light Color Absorbance 380 Ultraviolet .433 400 Violet .506 420 .572 430 .606 440 .593 460 .448 480 .382 500 .195 520 .145 540 .144 560 .129 580 .144 600 .150 620 .157 640 .159 660 .247 680 .161 700 .087 2. Make a graph of wavelength vs. absorbance for the above data. 3. If you only had the above data, what color would you say the substance represented by the spectrograph is? Why?
The electromagnetic radiation is characterized by its wavelength (represented by ) or by its frequency (v) and both are directly related to the velocity of light.Electromagnetic radiation spectrum ranges from high cosmic waves to low energy wave lengths.
From the above data values given, the graph of wave length vs absorbance of the sample shows that they fall under visible range with wavelengths from 400 nm to 700 nm.
The visible range wave lengths are as follows:
Blue color - wavelength ranges between 400 nm to 500 nm ( rays, x-rays and uv rays falls under this category). Shoter wavelengths falls uder UV ray category.
Green color - wave length between 500 nm to 600 nm
Red color - wave length between 600 nm to 700 nm (IR rays, microwave rays and radio waves). Longer wavelengths falls under infrared region.
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