Question

When you were setting your crosses, you may have noticed that the UAS-white-RNAi expressing flies had...

When you were setting your crosses, you may have noticed that the UAS-white-RNAi expressing flies had red eyes. Why does the RNAi not work in these flies?

How is the chi-square test used to analyze genetic crosses? What does the probability (p-value) associated with a chi-square value indicate about the result of a cross?

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Answer #2

1 In UAS- GAL4 system , GAL4 is the transcriptional activator which binds to the enhancer sequence UAS , then there is Transcription of the downstream coded genes like RNAi. If there is no GAL4 , there will be no Transcription. That's why RNAi not working here.

2. Chi square test is used to compare the observed values to the expected ones . And to know whether the difference observed is significant or not .

The p value indicates whether the difference is significant or not. When p value is 0.0 5 or less than it , the difference is significant.

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