Question

You believe that the axons required to form connections between the eye and the brain are guided by Wnt signaling excreted from the brain.

1.What are TWO ways you can demonstrate that Wnt signaling is involved the in the eye axons (retinal ganglion cells) reaching the brain region (optic lobe)

2.How could you verify the “canonical” pathway was required? (the pathway on the bottom)

3.How could you verify it was specifically the canonical pathway?

Wnt--> Frizzled/LRP5/6--> Disheveled--| GSK3--| B-catenin--> Transcription--->

Answer 1

1 by cononical wntpathway noncononical planar cell polarity pathwat and non canonical wnt calcium pathway 2 cononical Wnt pathways are involved in the control of gene expression, cell behavior, cell adhesion, and cell polarity. 3 the wnt signoling is activated via frizzled protiens and LRP5/6 upon ligation to their receptors the cytpplasmic protein disheveled is received phosphorylated and activation of dvl induces the dissociation of Gsk-3beta and leads to inhibition of gsk-3 beta next the phosphorylation and degradation of beta catenin is inhibitef as a result of the inactivation of destruction complex subsequently stabilized beta catenin translocates into nucleus leading to changes in different target gene expression ie transcription