Question

You have conducted a series of pilot scale experiments on a fed batch reactor for a novel bioproduct. You plan to scale up your reactor to 1000 L and harvest biomass every 10 hours. Your initial culture volume of 400 L has a glucose concentration (So) of 20 gL^-1. Glucose is added at a concentration of 200 g/L (SF). The initial biomass concentration is 50 gL^-1. The kinetic and yield factors of the organism are mmax = 0.4 h^-1, Ks = 0.5 g L^-1, and Y_x/s = 0.3 g-cell g-glucose^-1. Endogenous growth and maintenance are negligible (i.e., kd = 0) and you can use the pseudo-steady state assumption.

If at 10 hours your reactor is at 90% of capacity, what is the flow rate of nutrient entering the reactor?

What is the glucose concentration (g L^-1) after 10 hours?

How much glucose (g) has the organism consumed?

How much biomass (g) was produced?

Answer 1

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