You design an experiment to determine whether a chemical will affect the amount of peptidoglycan in a species of gram-positive bacteria. You classify each individual as either normal peptidoglycan (cell stains in a normal way), reduced peptidoglycan (cell stains but the stain is patchy), or absent (no stain). Under normal conditions, 10% of cells do not stain, 7% show patchy staining, and 83% stain normally. Test whether the chemical alters the amount of peptidoglycan from the expected proportions.
Normal = 145, reduced = 180, absent = 165
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