how to use excell to Using excel, You will need to plot the log of the copy number (x axis) against the CT values (Y-axis) and generate a line of best fit together with an R2 value (which measures the goodness of fit).
copies per UL | cq(Value) | |
10,000,000 | 28.87 | |
1,000,000 | 32.01 | |
100,000 | 35.27 | |
10,000 | 38.87 |
SUMMARY OUTPUT | ||||||
Regression Statistics | ||||||
Multiple R | 0.9995003 | |||||
R Square | 0.99900084 | |||||
Adjusted R Square | 0.99850127 | |||||
Standard Error | 0.16631296 | |||||
Observations | 4 | |||||
ANOVA | ||||||
df | SS | MS | F | Significance F | ||
Regression | 1 | 55.31138 | 55.31138 | 1999.688 | 0.000499703 | |
Residual | 2 | 0.05532 | 0.02766 | |||
Total | 3 | 55.3667 | ||||
Coefficients | Standard Error | t Stat | P-value | Lower 95% | Upper 95% | |
Intercept | 52.048 | 0.417442212 | 124.6831 | 6.43E-05 | 50.25189113 | 53.8441089 |
log (x) | -1.4444634 | 0.032301701 | -44.7179 | 0.0005 | -1.58344645 | -1.3054804 |
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