Question

If the microscope’s calibration factor is 100 nm / pixel, what dilution of beads do you need to make to achieve the desired density in the viewing area? The density of polystyrene is ≈ 1.05 g/ml. Assume that the depth of field ≈ 5 µm

Answer 1

Solution:

Caliberation factor= 100nm/ pioxel

density of polysterene = 1.05 g/ml

depth of field = 5 micro m

now to find the dilution of beads:

The following steps needed to be taken care of:

1. The medium between lens and sample is approx 1.5(NA =n sin \theta)

2. Uing n>1 and increasing microscope's resoltuion.

3. The next step is to convert the images of di using beads into trajectories (x_{n},y_{n}) for each bead, where (xn = (x(n*t)))

4. Now, Using the mean-square displacement we calculate the diffusion factor by the following formula: