Question

Describe how leading-edge and trailing-edge F

Describe how leading-edge and trailing-edge F

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Answer #1

These experiments would involve labelling a short DNA fragment (containing the appropriate promoter and initial transcribed region) with an acceptor fluorophore at either the downstream end (leading-edge) or upstream end (trailing-edge) of the template. The sigma factor would be labelled at one known position with a donor fluorophore. Initiate transcription allowing the RNAP to proceed to fixed position in the initial transcribed region of the DNA template while monitoring the FRET signal to determine if the sigma factor is released

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