Question

A total internal reflection fluorescence microscope uses a laser beam that undergoes total internal reflection within...

A total internal reflection fluorescence microscope uses a laser beam that undergoes total internal reflection within a glass microscope slide. The laser travels through a glass microscope slide and hits an interface with water. The index of refraction of water is 1.33 and the index of the glass is 1.52. What is the critical angle at which total internal reflection occurs within the microscope slide?

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