The Point Spread Function (PSF) plays an important role in the image formation theory of the fluorescence microscope. It is the main brick that builds up the whole acquired image.
Given data:
Mutual focal plane distance = 10 cm
So, distance between lens and N.A is 10 cm.
When first lens is placed on the focal plane of first lens i.e at 20 cm. = object distance =20 cm
The PSF of an optical device is the image of a single point object (rescaled to make its integral all over the space equal 1). The degree of spreading (blurring) in the image of this point object is a measure for the quality of an optical system.
As rays paases throguh NA it gives clear image .PSF will not be blur
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