Bring near (but not touching) some other charged object X to one of the two. The charge of the opposite sign to X will be attracted to the side near X. Because the spheres are insulated (and the air is dry) the reverse charge appears on (the far side of) the other sphere because the insulation prevents the two spheres from gaining or losing charge from elsewhere. Separate the two spheres and then move X away. The balanced charge difference remains trapped on the two spheres.
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