Question

Suppose the Förster distance of a pair of donor-acceptor fluorophores is estimated to be 5 nm....

Suppose the Förster distance of a pair of donor-acceptor fluorophores is estimated to be 5 nm. How much will the FRET efficiency be, if the two fluorophores are separated by 6 nm? Assume that the environmental conditions remain the same.

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Answer #1

The FRET efficiency E is the quantum yield of the energy-transfer transition, i.e. the probability of energy-transfer event occurring per donor excitation event.E depends on the donor-to-acceptor separation distance r with an inverse 6th-power law due to the dipole–dipole coupling mechanism:

r = 6nm (donor-acceptor fluorophores distance)

R= 5nm (Förster distance of this pair of fluorophores)

The FRET efficiency is 0.2509

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