Question

Suppose the Förster distance of a pair of donor-acceptor fluorophores is estimated to be 5 nm....

Suppose the Förster distance of a pair of donor-acceptor fluorophores is estimated to be 5 nm. How much will the FRET efficiency be, if the two fluorophores are separated by 6 nm? Assume that the environmental conditions remain the same.

Science   Advanced-Physics   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

The FRET efficiency E is the quantum yield of the energy-transfer transition, i.e. the probability of energy-transfer event occurring per donor excitation event.E depends on the donor-to-acceptor separation distance r with an inverse 6th-power law due to the dipole–dipole coupling mechanism:

r = 6nm (donor-acceptor fluorophores distance)

R= 5nm (Förster distance of this pair of fluorophores)

The FRET efficiency is 0.2509

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
Light of wavelength 532 nm illuminates a pair of slits separated by a distance of d=...
Light of wavelength 532 nm illuminates a pair of slits separated by a distance of d= 0.42 mm. An interference pattern is observed on a screen placed a distance L away (L>>d). You may use the small angle approximation for this problem. What is the distance L if the width of the central bright spot of the interference pattern is delta(y) = 1.9 cm? The answer is supposed to be 15 m but i cant figure out how to get...
A pair of antennae broadcast a signal in phase at 100MHz and are separated by a...
A pair of antennae broadcast a signal in phase at 100MHz and are separated by a distance of 6 m. At distances much greater than 6 m, in what directions listed below is the intensity from the two antennae greatest? The angles are defined such that 0o corresponds to the direction perpendicular to the line connecting the two antennae, as shown in the figure. We can assume that each of the antennae radiates uniformly in all directions. Group of answer...
Suppose rRF = 5%, rM = 10%, and bi = 1.8. What is ri, the required...
Suppose rRF = 5%, rM = 10%, and bi = 1.8. What is ri, the required rate of return on Stock i? Round your answer to two decimal places. % 1. Now suppose rRF increases to 6%. The slope of the SML remains constant. How would this affect rM and ri? Both rM and ri will decrease by 1%. Both rM and ri will remain the same. Both rM and ri will increase by 1%. rM will remain the same...
1. Find the distance between two slits that produces the first minimum for 543 nm violet...
1. Find the distance between two slits that produces the first minimum for 543 nm violet light at an angle of 40.4º. Give your answer in microns.v21b3 3yyyn 2. At what angle in degrees is the third-order maximum for 414 nm wavelength light falling on double slits separated by 0.0459 mm? 3. A soap bubble is 101 nm thick and illuminated by white light incident perpendicular to its surface. What wavelength in nanometers is most constructively reflected, assuming the same...
Suppose rRF = 6%, rM = 9%, and bi = 1.6. 2. Now suppose rRF decreases...
Suppose rRF = 6%, rM = 9%, and bi = 1.6. 2. Now suppose rRF decreases to 5%. The slope of the SML remains constant. How would this affect rM and ri? What is ri, the required rate of return on Stock i? Round your answer to two decimal places. % 1. Now suppose rRF increases to 7%. The slope of the SML remains constant. How would this affect rM and ri? Both rM and ri will increase by 1%....
REQUIRED RATE OF RETURN Suppose rRF = 6%, rM = 11%, and bi = 1.6. 2....
REQUIRED RATE OF RETURN Suppose rRF = 6%, rM = 11%, and bi = 1.6. 2. Now suppose rRF decreases to 5%. The slope of the SML remains constant. How would this affect rM and ri? What is ri, the required rate of return on Stock i? Round your answer to two decimal places. % 1. Now suppose rRF increases to 7%. The slope of the SML remains constant. How would this affect rM and ri? rM will remain the...
As long as the distance to the wall is much greater than the separation of the...
As long as the distance to the wall is much greater than the separation of the slits, the spacing between the bright spots is uniform. Using data from the Double Slit Experiment Data table, compute the distance between two bright spots. 3. How close together were you able to make your slits?  In other words, calculate the quantity d in the relation y = L/d .  Show all of your work. 4. If you were to use a green light instead of...
Suppose rRF = 6%, rM = 9%, and bi = 1.1. 2. Now suppose rRF decreases...
Suppose rRF = 6%, rM = 9%, and bi = 1.1. 2. Now suppose rRF decreases to 5%. The slope of the SML remains constant. How would this affect rM and ri? -Select-IIIIIIIVVItem 3 What is ri, the required rate of return on Stock i? Round your answer to one decimal place.   % 1. Now suppose rRF increases to 7%. The slope of the SML remains constant. How would this affect rM and ri? Both rM and ri will increase...
Suppose rRF = 4%, rM = 13%, and bi = 1.1. What is ri, the required...
Suppose rRF = 4%, rM = 13%, and bi = 1.1. What is ri, the required rate of return on Stock i? Round your answer to two decimal places. % 1. Now suppose rRF increases to 5%. The slope of the SML remains constant. How would this affect rM and ri? Both rM and ri will remain the same. Both rM and ri will increase by 1%. rM will remain the same and ri will increase by 1%. rM will...
You see a light approaching you from a distance on the highway. Is it a motorcycle...
You see a light approaching you from a distance on the highway. Is it a motorcycle (one light) or a car (two lights)? You may assume the air is perfectly clear and still, and the headlights emit exclusively blue light (λ = 475 nm). Assume your pupil (the aperture of your eye) is 5 mm in diameter. If a car with two blue headlights that are separated by 1.5 meters is approaching you, at what maximum distance could you still...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT