Question

Sketch a cuvette with 0.5cm, 1.0cm, 5.0 cm pathlength indicating with arrows what happens to the beam of light as it passes through each cuvette (assume you are using Cary60). Descirbe what is happening to the beam of light as it passes through the different cuvettes.

At wavelength 595nm

Answer 1

A cuvette is a small cubicle test tube which holds the sample solution. Thus the cuvette is then kept in the spectrometer to observe the sample. When the light passes through the solution, the particles in the solution diffracts the sample and only a few lights pass through it. The wavelength of light which is not observed is captured in the form of a graph.

so absorbance = E*C* L

that is

E = absorption coefficient

C = concentration of sample

L = optical path length

Thus for the cuvette whose path length is higher, the absorbance is higher. Hence, only a fraction of light comes out of the cuvette. For example, if the sample is a colored liquid, then all the wavelengths of the light are absorbed except the color of the liquid.

the beam of light through different cuvettes are,