Question

14) Consider the oxidation of glucose to carbon dioxide and water and the production of ATP

a. How many electrons pass through the mitochondrial electron transport chain per glucose molecule oxidized?

b. How many protons are pumped in the process? Remember that not all electrons go through NADH.

c. How much energy would be stored per mole of glucose by the resulting membrane potential at 37C (assume that the pH is 0.5 units lower on the outside than on the inside and that there is a membrane potential of 200 mV that is positive on the outside – protons are pumped out).

d. What is the efficiency of energy storage if you consider the process of glucose oxidation to the point of proton pumping (no ATP production yet)? Heat of combustion of glucose is -2805 kJ/mole

e. Based on these calculations, how many ATP molecules are made per glucose oxidized if you assume that protons pumped in the mitochondria are only used to make ATP in the AFTP synthetase (this will be different from what the book says – why? -think about the energy expenditure to get ATP out of the mitochrondria and phosphate in)?

f. Using the number you calculated above, what is the efficiency of energy storage as ATP from the proton gradient?

g. What is the overall efficiency of energy storage oxidizing glucose and making ATP (for this use the ATP numbers given in the book that account for both ATP transport and the ATP made by substrate level phosphorylation and assume ATP formation in the cell takes about 50 kJ/mole)?

Please show work for a-g thank you.

Answer 1