How to increase life-span of yeast after 36 hours and optimization for cell growth?
Calorie restriction (CR) has been shown to slow aging in evolutionarily divergent species, including yeast, worms, flies, and rodents . In addition to increasing longevity, CR is reported to cause additional phenotypes, including increased resistance to oxidative stress , enhanced DNA damage repair , decreased levels of oxidatively damaged proteins , improved glucose homeostasis and insulin sensitivity , altered levels of apoptosis , and delayed onset of a number of age-related diseases
In the yeast, CR can be imposed by reducing the concentration of glucose in the growth medium, resulting in a 20%–40% increase in replicative life span in multiple strain backgrounds [
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