Question

Problem1: The following data is available for Atropa belladonna, produced by transformation of plants Agrobacterium rhizogenes in a batch culture on sugar.

(i) Plot m as a function of culture time and average substrate concentration.

(ii) Calculate the specific growth rate at an average glucose concentration of 20 g/l and (iii) the maximum biomass concentration for 100 g/l sugar concentration

Time (d)	Biomass concentration, X (g /l)	Sugar concentration, S (g/l)
0	o.65	30.0
5	1.95	27.4
10	4.21	23.6
20	6.98	18.4
30	10.3	13.3
40	12.7	8.0
45	13.1	6.8
50	13.5	5.7
55	13.7	5.2

Answer 1

Ans:-

The procedure is as follows:-

a.

1. Enter the data in the spread sheet.

2. select the option 'Graph' from the main menu

3. select the option 'scatter plot'.

4. Specify the variables as 'Time' on X-axis and 'Biomass Conc' on Y-axis.

The graph is as shown below:

From the graph it is observed that it is an increasing function. As the time increases, the weight of biomass concentration is also increased.

b.

1. Enter the data in the spread sheet.

2. Select the option 'Graph' from the main menu

3. select the option 'scatter plot'.

4. Specify the variables as 'Time' on X-axis and 'sugar concentration' on Y-axis.