The loss of efficiency increases with the age of chromatographic column. An equation was derived by J. J. van Demeter that included the major factors contributing to this loss. With describing individual terms, he also derived a composite curve with related plate height (HETP) and the linear velocity of the mobile phase flowing through the column. For example, the figure below depicts the reduction in chromatographic performance sustained from system extra column.
Get Answers For Free
Most questions answered within 1 hours.