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Briefly describe when you would use the extraction (shake-flask) method vs. the LC-MS method to measure...

Briefly describe when you would use the extraction (shake-flask) method vs. the LC-MS method to measure LogP. Include considerations about sample size and polarity.

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Log P or Lipophilicity is expressed as octanol/water distribution coefficient (LogP), which is one of the important physicochemical parameter considererd in drug discovery and is used for the prediction of pharmacokinetic properties and intestinal absorption of drugs.

Calculation of Log P usinng LC-MS is used to calculate Lipophilic Efficiency (LipE) for the research, where the efficiency of the compound is defined as its potency with the help of a formula LipE = pIC50 – LogP/D. This method helps in the approximation of the interaction of the drug molecule in the cellular environment with the help of it's efficacy, how easily the drug is absorbed, how strong the effect is, how long it remains in the body in an active form, and how it is metabolized and excreted. LogP assay uses the octanol / water shake flask method for determining lipophilicity. LC-MS/MS is used to quantify the samples.

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