Question

The following data for the enzymatic hydrolysis of n-benzoyl 1-arginine ethyl ester (BAEE) by tripsin bound to particles of porous glass were obtained in a fixed bed reactor for an initial concentration CA0 = 0.5 mM.

XA | V/F'0, min |

0.438 | 5.90 x 10^-2 |

0.590 | 8.03 |

0.670 | 9.58 |

0.687 | 9.46 |

0.910 | 14.72 |

0.972 | 18.00 |

a. For Michaelis-Menten kinetics and assuming plug flow operation, show that a plot of (1/Ca0*XA) ln(1-XA)^-1 versus V/FA0*XA should give a straight line, from which the constants can be determined.

b. Compute values for the constants.

Answer #1

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