Question

The following data for the enzymatic hydrolysis of n-benzoyl 1-arginine ethyl ester (BAEE) by tripsin bound to particles of porous glass were obtained in a fixed bed reactor for an initial concentration CA0 = 0.5 mM.

XA	V/F'0, min
0.438	5.90 x 10^-2
0.590	8.03
0.670	9.58
0.687	9.46
0.910	14.72
0.972	18.00

a. For Michaelis-Menten kinetics and assuming plug flow operation, show that a plot of (1/Ca0*XA) ln(1-XA)^-1 versus V/FA0*XA should give a straight line, from which the constants can be determined.

b. Compute values for the constants.

Answer 1