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4. (6 pts) I have a new gene sequence, and I plan to do a PCR...

4. (6 pts) I have a new gene sequence, and I plan to do a PCR with 30 cycles for amplifying it. Since the sequence is rather long, I plan to use a high-fidelity DNA polymerase (i.e. one that has a very low error rate).
A) If the enzyme introduces an error in the 24th cycle, what will be the percentage of incorrect / erroneous products (3 pts)?

B) I made a mistake and added Taq DNA polymerase to my reaction mixture instead (which has a higher error rate). If the enzymes introduces an error in the 4th cycle, what will be the ratio of correct to incorrect products (3 pts)?

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