Question

The literature supplied by GE Healthcare, the manufacturer of Sephacryl resins, provides information concerning the fractionation...

The literature supplied by GE Healthcare, the manufacturer of Sephacryl resins, provides information concerning the fractionation range of the vaious S-type resins. This informaiton is presented in terms of the molecular weights of globular proteins and dextrans that can be fractionated by these columns. For instance, the fractionation range of Sephacryl S-300Hr is 10,000-1,500,00 for globular proteins and 2,000-400,000 for dextrans. Why does the fractionation range for globular proteins and dextrans differ?

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Answer #1

first let me clear about fractionation-

each and every types of resin have different structure,molecule present in it. so for different type of material have different structure so all have different fractionation range.

(here we are just represent information of fractionation in terms of molecular weight of 2 different material.)

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