Question

Fro each ion-exchange column (DEAE, CM), an alternative approach, other than the use of 1M KCl, for the eultion of tightly-bound sample components would be to change th epH of the elution buffer. Describe how this alternative approach would be carried out for each of the two columns and explain the basis for elution of the proteins under the altered conditions of elution. Conditions must be chosen where protein stability is likley to be maintained (buffers with pH values between 5 and 9).

Answer 1