Question

(8 pts) Calculate the melting temperature (Tm;°C) and the %GC content of the following primer sequences....

(8 pts) Calculate the melting temperature (Tm;°C) and the %GC content of the following primer sequences. Use the formula given in class for calculating Tm.

A) 5’-AGGAATCGCCGATGGCGACA-3’ (4 pts)

B) 5’-GGACTTTAAGAATCCTTGCGCATCCTGGAGGAGTCC-3’ (4 pts)

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