Question

You are working to characterize a newly discovered enzyme. Upon performing an enzyme kinetics assay, you receive the following data. Here this assay was performed by placing the purified enzyme with substrate and the formation of product was measured as a function of time. Plot this data on a Michaelis Menton style of plot. Plot this data on a Lineweaver Burk style of plot.

[S] (mmol/L) Vo (µmol/min) Vo (µmol/min) [10.0 µM inhibitor]

0.000 0.000 0.000

0.25 4.66 0.646

0.5 7.11 1.24

1.0   9.65 2.29

Answer 1

In a Michaelis-Menten plot, the reaction rate (V0) is plotted against the substrate concentration [S].

In a Lineweaver Burk Plot, the inverse of reaction rate (1/V0) is plotted against the inverse of the substrate concentration (1/[S]). The plot is a straight line.

#Without Inhibition

Data Table:

[S] (mmol/L)	V0 (umol/L)	1/[S]	1/V0
0	0
0.25	4.66	4	0.214592
0.5	7.11	2	0.140647
1	9.65	1	0.103627

#With Inhibition

Data Table:

[S] (mmol/L)	V0 (umol/L)	1/[S]	1/V0
0	0
0.25	0.646	4	1.547988
0.5	1.24	2	0.806452
1	2.29	1	0.436681