Question

Cells of Pichia pastoris yeast are cultured in medium containing glycerol (C3H8O3) as the carbon source and ammonium hydroxide as the nitrogen source. Under aerobic conditions, the yield of biomass from substrate is 0.57 g/g. Biomass, CO2, and H2O are the only major products of this culture.

(a) Estimate the cooling requirements per g of biomass produced.

(b) When all the glycerol has been consumed, methanol (CH4O) is added to the culture. There is enough ammonium hydroxide remaining in the medium to support growth of the biomass with methanol as substrate. The composition of the cells is essentially unchanged with a biomass yield from substrate of 0.44 g g21. In what way do the cooling demands change per g of biomass produced?

Answer 1

Sorry for the cutting