Question

Mass spectrometry (MS) provides a high sensitive method for identifying unknown proteins. Charged particles have very precise dynamics when subjected to electrical and magnetic fields in a vacuum. MS exploits this principle to separate ions according to their (10 pts):

Select one:

a. Hydrophobicity

b. Mass-to-charge ratio

c. Solubility

d. Radioactivity

Answer 1

Answer) b. Mass to charge ratio.

Proteins when subjected to electric field they move to positive voltage side it proteins is negative charge and vice versa, and does not move if neutral charge. Hence we can get idea of charge of protein by this.

Larger proteins will move slowly and will not be able to move larger distance , hence we can get idea of weight of protein by this.

Every protein has their unique mass to charge ratio. And by that we can find the unknown protein using mass spectrometry.

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