Question

Glucose (mg/mL)	Absorbance (600 nm)
0.0	0.000
0.1	0.150
0.2	0.300
0.3	0.450
0.4	0.600
0.5	0.750

You are provided with a patient’s serum sample. In order to determine the glucose concentration in the serum sample you dilute it by adding 0.20 mL of the serum sample to 0.80 mL of saline.

            Using the standard glucose assay, the absorbance reading for the diluted serum sample is 0.480.

            Determine the glucose concentration in the patient’s serum sample.

            State the glucose concentration in the serum sample in units of mg/mL and mmol/L:

Answer 1

ANSWER: The concentration of serum glucose at absorbance of 480 nm , from the serum sample of the patient is calculated to be 0.32 mg/mL.

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