Question

Which real-time PCR data curve provides information on the amplification efficiency of the reaction?

melting curve

cycle threshold

Tm

amplification curve

Answer 1

Real-time PCR data curve provides information on the amplification efficiency of the reaction-

amplification curve

Amplification efficiency is defined as-
The rate at which a PCR amplicon is generated, commonly measured as a percentage value. If a particular PCR amplicon doubles in quantity during the geometric phase of its
PCR amplification then the PCR assay is said to have 100% efficiency. The value assigned to the efficiency of a PCR reaction is a measure of the overall performance of a
real-time PCR assay