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unstained cells could be viewed with all of the following types of microscopy except?

unstained cells could be viewed with all of the following types of microscopy except?

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Answer #2

Darkfield microscopy is used to improve the contrast in unstained samples by using an illumination technique.
Phase-contrast microscopy is used to get contrast in a translucent specimen without staining the specimen.
Differential interference contrast microscopy is optical microscopy working on the principle of interferometry technique to enhance the contrast in unstained, transparent samples.

Fluorescence microscopy needs stains as it works by visualization of emitted fluorescence from the excitation light. For example, Rhodamine -is a protein-specific fluorescent stain used in fluorescence.

The answer is, Fluorescence microscopy

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