Question

Aya, a 1st year Nursing student was assigned to perform Vitamin Analysis in some fruits and vegetables. Her objective was to confirm if these fruits and vegetables really contain Vitamins A, B, C, D, and E. She used the following fruits/ vegetables: squash for Vitamin A, soy for Vitamin B3, orange for Vitamin C and tofu for Vitamin D.

What tests/ reagents will Aya use to detect the following Vitamins? Explain.

a. Test for Vitamin A

b. Test for Vitamin B3

c. Test for Vitamin C

d. Test for Vitamin D

Answer 1

a) CF3COOH inCH2CL2

b)2-amino-10-ethyl acridine ketone (AEAO), has been synthesized and successfully applied to the analysis of vitamin B3

c)Vitamin C Reagent (dichlorophenolindophenol)indicator solution is blue. A colorless end point will be reached when a solution containing vitamin C (such as orange juice) is added to this indicator.

d)The Diazyme EZ Vitamin D assay is intended for use in clinical laboratories for the quantitative determination of 25-OH vitamin D(vitamin D) in human serum and plasma, using automated chemistry analyzers. Measurement of vitamin Dis used for the assessment of thevitamin D sufficiency.